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Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 82-97 doi: 10.1007/s11783-011-0306-6

Abstract: Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.

Keywords： molecular biological technique     microbial community     denaturing gradient gel electrophoresis (DGGE)     terminalrestriction fragment length polymorphism (T-RFLP)     environmental applications    

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Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Frontiers of Environmental Science & Engineering 2009, Volume 3, Issue 2,   Pages 194-199 doi: 10.1007/s11783-009-0018-3

Abstract: This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis ofTwo kinds of tetrameric restriction enzymes, I and I, were used in the experiment to depict the bacterial

Keywords： terminal restriction fragment length polymorphism (T-RFLP)     bacterial community structure     marine microbial    

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Diversity and vertical distributions of sediment bacteria in an urban river contaminated by nutrients and heavy metals

Xunan YANG, Shan HUANG, Qunhe WU, Renduo ZHANG, Guangli LIU

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 6,   Pages 851-859 doi: 10.1007/s11783-013-0569-1

Abstract: The bacterial OTUs were measured using the terminal restriction fragment length polymorphism analysis

Keywords： heavy metals     nutrients     sediment profile     terminal restriction fragment length polymorphism    

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Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4,   Pages 559-567 doi: 10.1007/s11783-013-0501-8

Abstract: The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminalrestriction fragment length polymorphism (T-RFLP) methods.

Keywords： pyrene     bacterial communities     terminal restriction fragment length polymorphism     short-term exposure     rank-abundance    

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Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 92-98 doi: 10.1007/s11783-010-0254-6

Abstract: The community dynamics were investigated using specific PCR followed by terminal restriction fragment

Keywords： ammonia-oxidizing bacteria (AOB)     community dynamics     terminal restriction fragment length polymorphism    

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Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

Frontiers of Medicine 2018, Volume 12, Issue 6,   Pages 634-644 doi: 10.1007/s11684-018-0670-8

Abstract:

Calorie restriction (CR) is a dietary regimen that reduces calorie intake without incurring malnutrition

Keywords： caloric restriction     gut microbiota     metabolome    

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Selective epoxidation of linear terminal olefins with metalloporphyrins under mild conditions

Xiaoguang BAI, Yuanbin SHE

Frontiers of Chemical Science and Engineering 2009, Volume 3, Issue 3,   Pages 310-313 doi: 10.1007/s11705-009-0168-7

Abstract: The epoxidation of linear terminal olefins with metalloporphyrins in the presence of dioxygen and isobutyraldehyde

Keywords： metalloporphyrins     olefins     dioxygen     epoxidation    

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Corrigendum to “Research on DC protection strategy in multi-terminal hybrid HVDC System” [Engineering Corrigendum

Yuping Zheng, Jiawei He, Bin Li, Tonghua Wu, Wei Dai, Ye Li

Engineering 2022, Volume 10, Issue 3,   Pages 183-183 doi: 10.1016/j.eng.2022.02.006

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An identification method for enclosed voids restriction in manufacturability design for additive manufacturing

Shutian LIU,Quhao LI,Wenjiong CHEN,Liyong TONG,Gengdong CHENG

Frontiers of Mechanical Engineering 2015, Volume 10, Issue 2,   Pages 126-137 doi: 10.1007/s11465-015-0340-3

Abstract: In mathematics, enclosed voids restriction expressed as the solid structure is simply-connected.

Keywords： additive manufacturing     topology optimization     manufacturability constraints     design for additive manufacturing     simply-connected constraint    

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Accurate quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 240-250 doi: 10.1007/s11684-021-0909-7

Abstract: small RNAs, including microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), are modified at their 3′-terminalIn this study, we profiled 3′-terminal 2′-O-methyl plant miRNAs in the livers of rice-fed mice by oxidativeLikewise, stem-loop RT-qPCR was superior to poly(A)-tailed RT-qPCR in quantifying 3′-terminal 2′-O-methylIn summary, this study established a standard procedure for quantifying the levels of 3′-terminal 2′-Accurate measurement of the 3′-terminal 2′-O-methylation of small RNAs has profound implications for

Keywords： small RNAs     2′-O-methylation     sequencing     RT-qPCR    

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on the pollution control against wet weather overflow based on on-site coagulation/flocculation in terminal

Zongqun Chen, Wei Jin, Hailong Yin, Mengqi Han, Zuxin Xu

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 6, doi: 10.1007/s11783-021-1400-z

Abstract: The conventional overflow treatment based on coagulation/flocculation in terminal drainage systems requiresOn-site coagulation/flocculation in terminal drainage pipes was proposed in this study which was aimedto transfer the coagulation/flocculation process to the inside of pipes at the terminal drainage systemThis work provides scientific guidance to address wet weather overflow in terminal drainage pipes.

Keywords： Wet weather overflow     On-site coagulation/flocculation     Fast removal of particulate pollutants    

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Research on Rubber Tired Gantry Crane Scheduling of Port Container Terminal

Wei Zhong,Shen Jinsheng,Xiao Rongna,Zhang Zhiwen,Shi Dinghuan

Strategic Study of CAE 2007, Volume 9, Issue 8,   Pages 47-51

Abstract:  Port container terminal is much more emphasized than before as a node of container transportation At container terminal,  rubber tired gantry crane has already been a bottleneck of terminal This paper studies the rubber tired gantry crane scheduling of port container terminal in the quantitativecondition by using proposed mixed integer programming (MIP) model,  which gives decision support to terminal

Keywords： container terminal     yard     rubber tired gantry crane (RTGC)     scheduling    

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Research on DC Protection Strategy in Multi-Terminal Hybrid HVDC System Article

Yuping Zheng, Jiawei He, Bin Li, Tonghua Wu, Wei Dai, Ye Li

Engineering 2021, Volume 7, Issue 8,   Pages 1064-1075 doi: 10.1016/j.eng.2021.05.002

Abstract:

Multi-terminal hybrid high-voltage direct current (HVDC) systems have been developed quickly in recentconverter based HVDC (LCC-HVDC) systems or VSC-HVDC systems cannot distinguish the fault line in multi-terminalThis paper proposes a novel single-ended DC protection strategy suitable for the multi-terminal hybridprotection strategy is not dependent on the line boundary element and is thus suitable for the multi-terminal

Keywords： Multi-terminal hybrid HVDC system     Single-ended protection     Transient information     Active injection    

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The Development Trent of Advanced Communication Technology

Bi Houjie

Strategic Study of CAE 2000, Volume 2, Issue 8,   Pages 31-34

Abstract:

In this paper, the development trend of advanced communication network terminals is summarized and analyzed. It is instructive to point out that industrialization of communication software and development of the EDA technology must be strengthened in order to meet the challenge of entering WTO.

Keywords： telecommunication network     grouping     multimedium     mobility     communication terminal    

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Title Author Date Type Operation

Current molecular biologic techniques for characterizing environmental microbial community

Dawen GAO, Yu TAO

Journal Article

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG, Xiaolu JIANG, Ya HE, Huashi GUAN

Journal Article

Diversity and vertical distributions of sediment bacteria in an urban river contaminated by nutrients and heavy metals

Xunan YANG, Shan HUANG, Qunhe WU, Renduo ZHANG, Guangli LIU

Journal Article

Response of bacterial communities to short-term pyrene exposure in red soil

Jingjing PENG, Hong LI, Jianqiang SU, Qiufang ZHANG, Junpeng RUI, Chao CAI

Journal Article

Community dynamics of ammonia oxidizing bacteria in a full-scale wastewater treatment system with nitrification stability

Xiaohui WANG, Xianghua WEN, Hengjing YAN, Kun DING, Man HU

Journal Article

Calorie restriction and its impact on gut microbial composition and global metabolism

Xiaojiao Zheng, Shouli Wang, Wei Jia

Journal Article

Key technological innovations in the construction of the main terminal of Beijing Daxing International

Journal Article

Selective epoxidation of linear terminal olefins with metalloporphyrins under mild conditions

Xiaoguang BAI, Yuanbin SHE

Journal Article

Corrigendum to “Research on DC protection strategy in multi-terminal hybrid HVDC System” [Engineering

Yuping Zheng, Jiawei He, Bin Li, Tonghua Wu, Wei Dai, Ye Li

Journal Article

An identification method for enclosed voids restriction in manufacturability design for additive manufacturing

Shutian LIU,Quhao LI,Wenjiong CHEN,Liyong TONG,Gengdong CHENG

Journal Article

Accurate quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing

Journal Article

on the pollution control against wet weather overflow based on on-site coagulation/flocculation in terminal

Zongqun Chen, Wei Jin, Hailong Yin, Mengqi Han, Zuxin Xu

Journal Article

Research on Rubber Tired Gantry Crane Scheduling of Port Container Terminal

Wei Zhong,Shen Jinsheng,Xiao Rongna,Zhang Zhiwen,Shi Dinghuan

Journal Article

Research on DC Protection Strategy in Multi-Terminal Hybrid HVDC System

Yuping Zheng, Jiawei He, Bin Li, Tonghua Wu, Wei Dai, Ye Li

Journal Article

The Development Trent of Advanced Communication Technology

Bi Houjie

Journal Article